Air France-KLM dampened Italian hopes of a revival of talks to buy Alitalia, saying on Monday the matter was closed.
Air France-KLM withdrew a bid for Alitalia in late April after talks broke down with the Italian airline's unions.
Speculation of a deal to fold Alitalia into Europe's biggest airline to help save it from bankruptcy was rekindled several days ago when Italian Prime Minister Silvio Berlusconi said Air France-KLM would be a good partner, softening his earlier stance.
Air France-KLM Chief Executive Jean-Cyril Spinetta said however the "dossier is closed".
Spinetta made the remark on the sidelines of a company presentation, according to an airline spokesperson.
Berlusconi, who earlier this year called Air France-KLM's now-defunct takeover offer for Alitalia "unacceptable", said on June 3 he had discussed the fate of the Italian carrier with French President Nicolas Sarkozy at a meeting last week.
"Alitalia will need to find accords with international partners and... Air France could be a good solution," Berlusconi told a joint news conference with Sarkozy.
Since withdrawing its bid in April Air France-KLM has said restarting talks would be difficult.
Berlusconi's new conservative government last month launched the third attempt in 18 months to find a buyer for Alitalia, which loses more than 1 million euros a day.
Alitalia's future has become even more uncertain with the rise in the cost of fuel and a steady decline in bookings.